Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source

TitleCombined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source
Publication TypeJournal Article
Year of Publication2006
AuthorsTang, S., T. B. Krasieva, Z. Chen, and B. J. Tromberg
JournalJ Biomed Opt
Volume11
Pagination020502
Abstract

A 12-fs broadband (100-nm) source is used to combine multiphoton microscopy (MPM) and optical coherence tomography (OCT) in a single platform. An ultrafast Ti:sapphire laser simultaneously provides short pulses necessary for efficient MPM excitation and the broad bandwidth required for high-resolution OCT. Using 0.3-microm microspheres and a 63x, 0.95 numerical aperture objective, we demonstrate that MPM and OCT channels are coregistered with lateral resolution of approximately 0.5 microm and axial resolution of approximately 1.5 microm. Preliminary studies of a 3-D organotypic epithelial tissue model show that multiphoton images of fluorescence and second harmonic signals are derived from cellular and extracellular matrix structures, respectively, while OCT images are generated from scattering interfaces due to tissue variations in refractive index. The combined MPM/OCT microscope is capable of providing simultaneous functional and structural information from cells and extracellular matrix and is potentially a powerful tool for studying biological processes in thick tissues.

URLhttp://dx.doi.org/10.1117/1.2193428
DOI10.1117/1.2193428

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